sunbet(中国)

    Hematoxyllin-Eosin ( HE ) Staining

    Freda Halim

    Published: 2023-03-20 DOI: 10.17504/protocols.io.4r3l27zo3g1y/v1
    AI 解读
    浏览 1345

    Disclaimer

    DISCLAIMER – FOR INFORMATIONAL PURPOSES ONLY; USE AT YOUR OWN RISK

    The protocol content here is for informational purposes only and does not constitute legal, medical, clinical, or safety advice, or otherwise; content added to protocols.io is not peer reviewed and may not have undergone a formal approval of any kind. Information presented in this protocol should not substitute for independent professional judgment, advice, diagnosis, or treatment. Any action you take or refrain from taking using or relying upon the information presented here is strictly at your own risk. You agree that neither the Company nor any of the authors, contributors, administrators, or anyone else associated with protocols.io, can be held responsible for your use of the information contained in or linked to this protocol or any of our Sites/Apps and Services.

    Abstract

    This is a protocol to stain tissue with Hematoxyllin-Eosin ( HE ) Staining. We use human breast cancer tissue for running this protocol.

    Steps

    Deparaffinization

    1.

    Incubate slides in Xylol

    2.

    Incubate slides in Xylol

    Rehydration

    3.

    Incubate slides in 100% Alcohol

    4.

    Incubate slides in 96% Alcohol

    5.

    Incubate slides in 80% Alcohol

    6.

    Incubate Slides in 70% Alcohol

    7.

    Rinse slides with Aquadest for 5 minutes

    Nuclear Staining

    8.

    Incubate Slides in Hematoxyllin Reagent ( Hematoxyllin JT Baker )

    9.

    Rinse slides with running tap water

    Defferentiation

    10.

    Incubate slides in bluing reagent @ 1 minutes, 5 times

    11.

    Rinse slides with running tap water

    Cytoplasm staining

    12.

    Incubate slides in Eosin reagent 1 minute

    Dehydration

    13.

    Incubate Slides in 70% Alcohol

    14.

    Incubate Slides in 80% Alcohol

    15.

    Incubate Slides in 96% Alcohol

    16.

    Incubate Slides in 100% Alcohol

    Clearing

    17.

    Incubate Slides in Xylol

    18.

    Incubate slides in Xylol

    19.

    Incubate Slides in Xylol

    Mounting and Finishing

    20.

    Wipe excess fluid,cover the slides with cover glass

    推荐阅读

    Nature Protocols
    Protocols IO
    Current Protocols
    扫码咨询