NCEM Drop - Inactivation of sample on grid (TM-014)
sandra.crameri
Published: 2023-05-13 DOI: 10.17504/protocols.io.8epv59mr6g1b/v1
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Abstract
NCEM Drop - inactivation of samples on grid
Steps
1.
Adsorb 10µL sample to grid 0h 10m 0s
2.
Immerse grid in drop of 2.5% volume 0.1Molarity (M) Sorenson's phosphate buffer 0h 10m 0s
3.
Wash 0.1Molarity (M) Sorenson's phosphate buffer
4.
Using filter paper wick excess buffer from grid - do not dry out, leave grid surface wet.
5.
Stain 0h 1m 0s
6.
Using filter paper gradually wick stain dry from grid
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