Preparation of BG-11 media Agar
Ashwinuday
Published: 2021-10-18 DOI: 10.17504/protocols.io.by6rpzd6
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Abstract
This is for making solid media of BG-11
Steps
1.
To make BG-11 Agar plates, add Agar-Agar Type-1 equivalent to 1.5% (w/v) of liquid media and autoclave.
2.
Then before it solidifies (when the flask is bearable to touch), add the required antibiotics to the media.
3.
Pour ~30-40 ml into plates soon after.
4.
Keep the plates opened in the hood.
5.
Once it solidifies in the plates then close those and keep the plates inverted into a cold storage.
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